Human CD300f/LMIR3 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF2774

R&D Systems, part of Bio-Techne
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AF2774
AF2774-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Cell Culture, Flow Cytometry, Immunoprecipitation, Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all CD300f/LMIR3 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD300f/LMIR3
Tyr16-Leu155 (Val19Ala)
Accession # Q8TDQ1

Specificity

Detects human CD300f/LMIR3 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) LMIR5 and rhLMIR4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human CD300f/LMIR3 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human CD300f/LMIR3

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD300f/LMIR3

CD300f, also known as CD300LF, LMIR3, IREM-1, CLM-1, IgSF13, DIgR1, and MAIR-V, is a 50‑60 kDa glycoprotein member of the immunoglobulin superfamily (1). Human CD300f consists of a 137 amino acid (aa) extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane segment, and a 113 aa cytoplasmic domain that contains multiple immunoreceptor tyrosine-based inhibitory motifs (ITIMs) or ITIM-like sequences (2, 3). Alternate splicing generates additional isoforms that carry substituted C-terminal tails of varying lengths and sequences following the ECD or transmembrane segment (3). Within the ECD, human CD300f shares 43% aa sequence identity with mouse and rat CD300f. CD300f is expressed on the surface of dendritic cells, monocytes, granulocytes, and mast cells as well as on acute myeloid leukemia (AML) blasts (2‑4). Pervanadate treatment or antibody crosslinking of CD300f induces phosphorylation of tyrosine residues in the cytoplasmic domain and the subsequent recruitment of phosphatases SHIP, SHP-1, SHP-2, and the p85 alpha subunit of PI3K (2, 3, 5, 6). CD300f ligation can induce cell death and inhibit signaling through multiple receptors including Fc epsilon RI, LMIR4, SCF R, TLR2, TLR3, and TLR9 (3‑8). In contrast, it enhances TLR4-mediated signaling/cytokine production in mast cells through association with the activating signaling protein FcR gamma (5). In mouse, a splice variant of CD300f (known as DIgR2, with a 7 aa insertion in the ECD) inhibits CD4+ T cell activation and in vivo Th1 and CTL responses (9). CD300f is up‑regulated on monocytes surrounding experimentally-induced spinal cord demyelination and functions as a negative regulator of inflammation in the CNS (10).

References

  1. Clark, G.J. et al. (2009) Trends Immunol. 30:209.
  2. Sui, L. et al. (2004) Biochem. Biophys. Res. Commun. 319:920.
  3. Alvarez-Errico, D. et al. (2004) Eur. J. Immunol. 34:3690.
  4. Korver, W. et al. (2009) Leukemia 23:1587.
  5. Izawa, K. et al. (2009) J. Immunol. 183:925.
  6. Alvarez-Errico, D. et al. (2007) J. Immunol. 178:808.
  7. Can, I. et al. (2008) J. Immunol. 180:207.
  8. Izawa, K. et al. (2007) J. Biol. Chem. 282:17997.
  9. Shi, L. et al. (2006) Blood 108:2678.
  10. Xi, H. et al. (2010) J. Exp. Med. 207:7.

Long Name

Leukocyte Mono Ig-like Receptor 3

Alternate Names

CD300f, CD300LF, CLIM1, CLM1, DIgR2, IGSF13, IREM-1, LMIR3, NKIR, Pigr3

Entrez Gene IDs

146722 (Human); 246746 (Mouse)

Gene Symbol

CD300LF

UniProt

Q8TDQ1

Additional CD300f/LMIR3 Products

Product Documents for Human CD300f/LMIR3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human CD300f/LMIR3 Antibody

For research use only

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