Human CD39L3/ENTPD3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4400
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD39L3/ENTPD3
Gln44-Pro485
Accession # O75355
Gln44-Pro485
Accession # O75355
Specificity
Detects human CD39L3/ENTPD3 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse CD39L3 is observed, and less than 2% cross-reactivity with recombinant human (rh) CD39L1 and rhCD39 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human CD39L3/ENTPD3 Antibody
CD39L3/ENTPD3 in Human Salivary Gland.
CD39L3/ENTPD3 was detected in immersion fixed paraffin-embedded sections of human salivary gland using Sheep Anti-Human CD39L3/ENTPD3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4400) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membranes of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
CD39L3/ENTPD3 in Human Pancreas.
CD39L3/ENTPD3 was detected in immersion fixed paraffin-embedded sections of human pancreas using Sheep Anti-Human CD39L3/ENTPD3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4400) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to pancreatic islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human CD39L3/ENTPD3 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human salivary gland and human pancreas
Sample: Immersion fixed paraffin-embedded sections of human salivary gland and human pancreas
Western Blot
0.1 µg/mL
Sample: Recombinant Human CD39L3/ENTPD3 (Catalog # 4400-EN)
Sample: Recombinant Human CD39L3/ENTPD3 (Catalog # 4400-EN)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD39L3/ENTPD3
Ectonucleoside triphosphate diphosphohydrolase-3 (NTPDase-3), encoded by the ENTPD3 gene and also known as CD39L3, is an integral membrane protein with an extracellular active site (1). Recombinant human NTPDase-3 was expressed as a protein lacking its N- and C-terminal transmembrane domains, resulting in the secretion of the soluble ectodomain. NTPDase-3 hydrolyzes the beta- and gamma-phosphate residues of nucleotides, preferring ATP, ADP, UTP, and UDP as substrates (1). Through its hydrolysis of extracellular nucleotides, NTPDase-3 plays a role in the regulation of purinergic signaling (2). The enzyme is expressed at its highest levels in brain, pancreas, spleen and prostate tissues (3).
References
- Lavoie, E.G. et al. (2004) Biochem. Pharmacol. 67:1917.
- Crawford, P.A. et al. (2007) Arch. Biochem. Biophys. 457:7.
- Chadwick, B.P. and A.M. Frischauf (1998) Genomics 50:357.
Long Name
Ecto-Nucleoside Triphosphate Diphosphohydrolase 3
Alternate Names
ATPDase, CD39L3, ENTPD3, HB6, NTPDase-3
Gene Symbol
ENTPD3
UniProt
Additional CD39L3/ENTPD3 Products
Product Documents for Human CD39L3/ENTPD3 Antibody
Product Specific Notices for Human CD39L3/ENTPD3 Antibody
For research use only
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