Human CD39L4/ENTPD5 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5297
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 743512
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human CD39L4/ENTPD5
Val21-His428
Accession # O75356
Val21-His428
Accession # O75356
Specificity
Detects human CD39L4/ENTPD5 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) CD39, rhCD39L1, or rhCD39L3 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human CD39L4/ENTPD5 Antibody
Detection of Human CD39L4/ENTPD5 by Western Blot.
Western blot shows lysates of human liver tissue and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human CD39L4/ENTPD5 Monoclonal Antibody (Catalog # MAB5297) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CD39L4/ENTPD5 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human CD39L4/ENTPD5 Antibody
Application
Recommended Usage
Western Blot
0.2 µg/mL
Sample: Human liver tissue and HepG2 human hepatocellular carcinoma cell line
Sample: Human liver tissue and HepG2 human hepatocellular carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD39L4/ENTPD5
CD39L4, also called ENTPD5 (ectonucleoside triphosphate diphosphohydrolase 5), ER-UDPase and PCPH, is a secreted, 45-51 kDa class II member of the GDA1/CD39 NTPase family of molecules. It is expressed by multiple cell types, including neurons, cardiomyocytes, and hepatocytes. CD39L4 is a divalent cation‑dependent enzyme that preferentially hydrolyzes GDP and UDP. In the ER, this enzyme may remove UDP which interferes with proper protein folding and maturation. Extracellularly, CD39L4 may reduce available UDP ligand for P2Y receptors. Mature human CD39L4 is a 408 amino acid (aa) glycoprotein (aa 21-428). It contains four APR (apyrase conserved regions) (aa 54-206) that participate in NTPase activity. CD39L4 has been reported to exist as both monomer and homodimer. Three potential splice variants are reported. One shows a seven aa substitution for aa 401-428, a second contains a five aa substitution for aa 401-428, and a third possesses a 22 aa substitution for aa 215-428. Over aa 21-428, human CD39L4 shares 89% aa sequence identity with mouse CD39L4.
Long Name
Ecto-Nucleoside Triphosphate Diphosphohydrolase 5
Alternate Names
CD39L4, ENTPD5, ER-UDPase, NTPDase 5, PCPH
Gene Symbol
ENTPD5
UniProt
Additional CD39L4/ENTPD5 Products
Product Documents for Human CD39L4/ENTPD5 Antibody
Product Specific Notices for Human CD39L4/ENTPD5 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...