Human CD3 epsilon mFluor™ Violet 450-Conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB100MFV450

Clone UCHT1 was used by HLDA to establish CD designation
R&D Systems, part of Bio-Techne
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FAB100MFV450-100
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

mFluor Violet 450 SE (Excitation = 406 nm, Emission = 445 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # UCHT1

View all CD3 epsilon Antibodies »

Product Specifications

Immunogen

Human thymocytes (1)

Specificity

Recognizes the epsilon-chain of the CD3/T cell antigen receptor complex.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CD3 epsilon mFluor™ Violet 450-Conjugated Antibody

Detection of CD3 epsilon in PBMC lymphocytes by Flow Cytometry.

PBMC lymphocytes were stained with Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Mouse Anti-Human CD3 epsilon mFluor™ Violet 450-Conjugated Monoclonal Antibody (Catalog # FAB100MFV450 or (B) unstained. View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD3 epsilon mFluor™ Violet 450-Conjugated Antibody

Application
Recommended Usage

Flow Cytometry

5 µL/106 cells
Sample: PBMC lymphocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD3 epsilon

CD3 epsilon is a key signaling molecule that participates in the formation of the T Cell Receptor (TCR). The alpha beta TCR is composed of six distinct subunits. There is an alpha-beta disulfide-linked heterodimer that recognizes antigen, and this is accompanied by four CD3 subunits that transduce an intracellular signal. CD3 epsilon forms a heterodimer with both CD3 gamma and CD3 delta, creating two signaling complexes. These two heterodimers are supplemented by a CD3 zeta zeta homodimer that lies in the center of the TCR complex, generating a fully-functional TCR (2). 

References

  1. Beverly, P.C.L. and R.E. Callard (1981) Eur. J. Immunol. 11:329.
  2. Call, M.E. and K.W. Wucherpfennig (2005) Annu. Rev. Immunol. 23:101.
  3. McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York.
  4. Knapp, W. et al. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York.
  5. Schlossman, S. et al. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York.

Alternate Names

CD3e

Entrez Gene IDs

916 (Human); 12501 (Mouse); 102133065 (Cynomolgus Monkey)

Gene Symbol

CD3E

Additional CD3 epsilon Products

Product Documents for Human CD3 epsilon mFluor™ Violet 450-Conjugated Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CD3 epsilon mFluor™ Violet 450-Conjugated Antibody

mFluorTM is a trademark of AAT Bioquest, Inc.

For research use only

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