Human CD58/LFA-3 Alexa Fluor® 647-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB1689R
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 248310
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD58/LFA‑3
Phe29-Arg215
Accession # P19256
Phe29-Arg215
Accession # P19256
Specificity
Detects human CD58/LFA‑3 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human CD58/LFA-3 Alexa Fluor® 647-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
0.25-1 µg/106 cells
Sample: Human whole blood monocytes
Sample: Human whole blood monocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CD58/LFA-3
T cells require a signal induced by the engagement of the T cell receptor and a “co-stimulatory” signal(s) through distinct T cell surface molecules for optimal T cell expansion and activation. Many cell-bound receptor-ligand pairs have now been shown to be involved in T cell co-stimulation including CD58/CD2 in humans and CD48/CD2 in mice and rats. CD58, also known as lymphocyte function-associated antigen (LFA-3), is a 210 amino acid protein that belongs to the CD2 family of the immunoglobulin superfamily (1). CD58 is widely expressed on hematopoietic and non-hematopoietic human tissue and has been found on leukocytes, erythrocytes, endothelial cells, epithelial cells and fibroblasts of human origin (2). No mouse or rat homolog of CD58 has as of yet been identified. CD58 has only one known ligand, CD2. CD2 is expressed on T cells, NK cells and dendritic cells (2‑4). CD2 ligation by CD58 has been shown to mediate T cell adhesion, T cell activation, T cell cytokine production and T cell and NK cells cytotoxic activity (1, 3, 5, 6). In dendritic cells, CD2 engagement increases MHC Class II, CD40, CD80, CD86, CD58 and CCR7 and induces IL-1 beta and IL-12 cytokine secretion (4).
References
- Davis, S.J. and P.A. van der Merwe (1996) Immunol. Today 17:177.
- Smith, M.E. and J.A. Thomas (1990) J. Clin. Pathol. 43:893.
- Bolhuis, R.L. Roozemond, R.C. and R.J. van de Griend (1986) J. Immunol. 136:3939.
- Crawford, K. et al. (2003) Blood 102:1745.
- Kanner, S.B. et al. (1992) J. Immunol. 148:2023.
- Bullens, M.A. et al. (2001) International Immunol. 12:181.
Alternate Names
CD58, LFA-3, LFA3
Gene Symbol
CD58
UniProt
Additional CD58/LFA-3 Products
Product Documents for Human CD58/LFA-3 Alexa Fluor® 647-conjugated Antibody
Product Specific Notices for Human CD58/LFA-3 Alexa Fluor® 647-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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