Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Blockade of Receptor-ligand Interaction, CyTOF-ready, Flow Cytometry

Cited:

Flow Cytometry, Functional Assay, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 131412

View all CD94 Antibodies »

Product Specifications

Immunogen

BaF3 mouse pro-B cell line transfected with human CD94 and NKG2A

Specificity

Recognizes human CD94 both in its homodimeric form and as a heterodimer with either NKG2A or NKG2C.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications for Human CD94 Antibody

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

HLA-E tetramers at 3 μg/mL were incubated with BaF/3 cells transfected with hNKG2C/CD94, and binding was monitored by flow cytometry. A 15 minute pre-incubation of the transfected cells with this antibody at a concentration of 17 μg/mL inhibited 95% of the HLA-E tetramer binding.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human whole blood CD56+ natural killer cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 2 using MAB1058 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD94

The type-II transmembrane glycoprotein CD94 covalently associates with C-type lectins of the NKG2 family to yield heterodimers important in NK cell recognition of class I MHC molecules. CD94/NKG2A complexes are also found on a subset of CD8+ T cells. Expression of CD94/NKG2 heterodimers may regulate cell survival and effector functions. There are two alternatively spliced variants of CD94 that differ by 31 amino acids encoded by exon 2.

Alternate Names

CD94, KLRD1

Entrez Gene IDs

3824 (Human); 16643 (Mouse); 102119829 (Cynomolgus Monkey)

Gene Symbol

KLRD1

Additional CD94 Products

Product Documents for Human CD94 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CD94 Antibody

For research use only

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