Human CDK5 Activator 1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7250
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CDK5 Activator 1
Gln100-Arg307
Accession # Q15078
Gln100-Arg307
Accession # Q15078
Specificity
Detects human CDK5 Activator 1 in direct ELISAs.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human CDK5 Activator 1 Antibody
CDK5 Activator 1 in Human Brain.
CDK5 Activator 1 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Sheep Anti-Human CDK5 Activator 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7250) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to nuclei in neuronal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human CDK5 Activator 1 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CDK5 Activator 1
Cyclin dependent kinase 5 regulatory subunit 1 (CDK5R1) is a 35 kDa protein that associates with and activates CDK5. Its expression is restricted to neurons and is required for normal brain development. CDK5R1 can be cleaved by calpain at Phe98-Ala99 which liberates a C-terminal fragment known as p25. p25 induces prolonged activation of CDK5 and broadens the substrate specificity of CDK5 to include tau which is aberrantly phosphorylated in Alzheimer's disease. Full length CDK5R1 is myristoylated at Gly2 and is preferentially localized to membranes at the cell periphery. p25 lacks this modification and is enriched in the nucleus and perinuclear regions. p25 accumulates in the brains of Alzheimer's patients where it promotes neuronal apoptosis and cytoskeletal abnormalities. Within aa 100-307, human CDK5R1 shares 99% aa sequence identity with mouse and rat CDK5R1.
Long Name
Cyclin-dependent Kinase 5, Regulatory Subunit 1 (p35)
Alternate Names
CDK5P35, CDK5R1, NCK5A, Neuronal CDK5 Activator, p35nck5a, TPKII Regulatory Subunit
Gene Symbol
CDK5R1
UniProt
Additional CDK5 Activator 1 Products
Product Documents for Human CDK5 Activator 1 Antibody
Product Specific Notices for Human CDK5 Activator 1 Antibody
For research use only
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