Human CEACAM-19 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9397

R&D Systems, part of Bio-Techne
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MAB9397-100
MAB9397-SP
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1032215

View all CEACAM-19 Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell HEK293-derived human CEACAM-19
Ala33-Gly157
Accession # Q7692-1

Specificity

Detects human CEACAM-19 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CEACAM-19 Antibody

Detection of CEACAM‑19 in HEK293 Human Cell Line Transfected with Human CEACAM-19 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) human CEACAM-19 or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human CEACAM-19 Monoclonal Antibody (Catalog # MAB9397) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB0041).Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human CEACAM-19 Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human CEACAM-20 and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEACAM-19

Carcinoembryonic antigen-related cell adhesion molecule-19 (CEACAM-19), also known as CEA-like gene 1 (CEAL1), is a member of the CEACAM subfamily of glycoproteins in the immunoglobulin (Ig) superfamily. Mature human CEACAM-19 consists of a 125 amino acid (aa) extracellular domain, a 21 aa helical transmembrane domain, and a 122 aa cytoplasmic domain (1,2). The extracellular domain contains an Ig-like domain as well as a glycosylation site, while the cytoplasmic domain is predicted to contain at least one immunoreceptor tyrosine-based activation motif (ITAM) (3,4). The extracellular domain of human CEACAM-19 shows 71%, 72%, and 78% aa identity to mouse, rat, and bovine CEACAM-19. The CEACAM family of proteins are involved in numerous intercellular-adhesion and intracellular signaling processes including cell adhesion, cell growth, recognition and differentiation, angiogenesis, and apoptosis (5,6). CEACAM-19 expression has been identified in a wide range of tissues including prostate, uterus, fetal brain, mammary and adrenal glands, skeletal muscle, small intestine, and kidney (1). Human CEACAM-19 has been found to be overexpressed in BT-474, BT20, and T47D breast cancer cell lines as well as LNCaP prostate cancer cells, suggesting a role in tumor progression and metastasis (1,3, 7). The overexpression in breast cancer patients is associated with poor prognosis (7).

References

  1. Schorilas, A. et al. (2003) Gene 310:79.
  2. Estiar, M. et al. (2016) Clin Exp Med doi:10.1007/s10238-016-0442-1.
  3. Beauchemin, N. and Arabzadeh, A. (2013) Cancer Metastasis Rev 32 (3-4):643.
  4. Kuespert, K. et al. (2006) Curr Opin Cell Biol 18:565.
  5. Obrink, B. (1997) Curr Opin Cell Biol 9:616.
  6. Horst, AK. and Wagener, C. (2004) Handb Exp Pharmacol 283.
  7. Michaelidou, K. et al. (2013) Int J Oncol 42:1770.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 19

Alternate Names

CEACAM19, CEAL1

Entrez Gene IDs

56971 (Human); 319930 (Mouse); 680640 (Rat)

Gene Symbol

CEACAM19

UniProt

Q7692-1

Additional CEACAM-19 Products

Product Documents for Human CEACAM-19 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human CEACAM-19 Antibody

For research use only

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