Human CEACAM-3/CD66d Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF4166
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Western Blot
Label
Biotin
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CEACAM‑3/CD66d isoform 1
Lys35-Gly155
Accession # P40198
Lys35-Gly155
Accession # P40198
Specificity
Detects human CEACAM‑3/CD66d in Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human (rh) CEACAM-1 is observed, 10% cross-reactivity with rhCEACAM-6 is observed, and 5% cross-reactivity with rhCEACAM-4 and rhCEACAM‑5 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human CEACAM-3/CD66d Biotinylated Antibody
Detection of CEACAM-3/CD66d in Human Granulocytes by Flow Cytometry.
Human whole blood granulocytes were stained with Sheep Anti-Human CEACAM-3/ CD66d Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4166, filled histogram) or control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).Applications for Human CEACAM-3/CD66d Biotinylated Antibody
Application
Recommended Usage
Flow Cytometry
2.5 µg/106 cells
Sample: Human whole blood granulocytes
Sample: Human whole blood granulocytes
Western Blot
0.1 µg/mL
Sample: Recombinant Human CEACAM-3/CD66d
Sample: Recombinant Human CEACAM-3/CD66d
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CEACAM-3/CD66d
CEACAM-3 (carcinoembryonic antigen-related cell adhesion molecule 3; also CD66d and CGM1) is a 31‑34 kDa member of the CEACAM subfamily of the CEA family of proteins. It is expressed by neutrophils and serves as a microbial receptor for a wide range of microorganisms. Mature human CEACAM-3 is a 218 amino acid (aa) type I transmembrane glycoprotein. Its extracellular domain (aa 35‑155) shows one V-type Ig-like domain (aa 35‑142). There are two alternate splice forms. One shows a 40 aa substitution for the C-terminal 116 amino acids (aa 137‑252), while a second shows a 31 aa substitution for the C-terminal 71 amino acids. No definitive rodent CEACAM-3 has been reported.
Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 3
Alternate Names
CD66d, CGM1
Gene Symbol
CEACAM3
UniProt
Additional CEACAM-3/CD66d Products
Product Documents for Human CEACAM-3/CD66d Biotinylated Antibody
Product Specific Notices for Human CEACAM-3/CD66d Biotinylated Antibody
For research use only
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