Human CELSR3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7278
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CELSR3
Tyr531-Ser711
Accession # Q9NYQ7
Tyr531-Ser711
Accession # Q9NYQ7
Specificity
Detects human CELSR3 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CELSR1 and rhCELSR2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human CELSR3 Antibody
Detection of CELSR3 in SH‑SY5Y Human Cell Line by Flow Cytometry.
SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human CELSR3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7278, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).Applications for Human CELSR3 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: SH‑SY5Y human neuroblastoma cell line
Sample: SH‑SY5Y human neuroblastoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CELSR3
CELSR3 (Cadherin EGF LAG Seven-pass G-type Receptor 3; also cadherin family member 11/CDHF11, FMI1 and EGFL1) is a 355 kDa (predicted) member of the LN-7TM subfamily, GPCR 2 family of proteins. It is coexpressed with Fzd3 on postmigratory neurons of the developing DRG and cranial ganglia, and appears to serve as an axonal guidance cue. Mature human CELSR3 is 3280 amino acids (aa) in length (aa 33-3312). It is a highly complex 7-transmembrane protein that contains a 2508 aa extended N-terminal extracellular region (aa 33-2540) plus a 538 aa C-terminal cytoplasmic domain. The N-terminal region contains nine consecutive cadherin domains (aa 326-1265), followed by a mixture of eight EGF-like and three laminin-like domains, and one membrane-proximal GPS domain. There are two potential isoform variants. One shows a five aa insertion after Gly2158, while another possesses an alternative start site at Met276. Over aa 531-711, human CELSR3 shares 97% aa sequence identity with mouse CELSR3.
Long Name
Cadherin, EGF LAG Seven-pass G-type Receptor 3
Alternate Names
CDHF11, EGFL1, HFMI1, MEGF2, RESDA1
Gene Symbol
CELSR3
UniProt
Additional CELSR3 Products
Product Documents for Human CELSR3 Antibody
Product Specific Notices for Human CELSR3 Antibody
For research use only
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