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Human Cerberus 1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1075

R&D Systems, part of Bio-Techne
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MAB1075
MAB1075-SP

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 162836

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Cerberus 1
Thr18-Ala267 (predicted)
Accession # O95813

Specificity

Detects human Cerberus 1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) DAN, rhGremlin, or recombinant chicken Caronte is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Cerberus 1 Antibody

Cerberus 1 antibody in Human Liver by Immunohistochemistry (IHC-P).

Cerberus 1 in Human Liver.

Cerberus 1 was detected in immersion fixed paraffin-embedded sections of human liver using 25 µg/mL Mouse Anti-Human Cerberus 1 Monoclonal Antibody (Catalog # MAB1075) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows secondary antibody only control experiment. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Cerberus 1 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Western Blot

1 µg/mL
Sample: Recombinant Human Cerberus 1

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cerberus 1

Cerberus 1 functions during development by binding to and antagonizing some members of the bone morphogenetic protein (BMP) family.

Alternate Names

CER1, DAND4

Entrez Gene IDs

9350 (Human); 12622 (Mouse)

Gene Symbol

CER1

UniProt

Additional Cerberus 1 Products

Product Documents for Human Cerberus 1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Cerberus 1 Antibody

For research use only

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