Human Chitotriosidase/CHIT1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB35591
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
ELISA Development
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG3 Clone # 397513
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Chitotriosidase/CHIT1
Ala22-Asn466
Accession # Q13231
Ala22-Asn466
Accession # Q13231
Specificity
Detects human Chitotriosidase/CHIT1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CHI3-L1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3
Applications for Human Chitotriosidase/CHIT1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human Chitotriosidase/CHIT1 (Catalog # 3559-GH)
Sample: Recombinant Human Chitotriosidase/CHIT1 (Catalog # 3559-GH)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Chitotriosidase/CHIT1
Chitotriosidase encoded by the CHIT1 gene is a member of the chitinase family that is selectively expressed in activated tissue macrophages (1). It is distinct from another member, known as acidic mammalian chitinase (AMC) encoded by the CHIA gene and expressed mainly in the gastrointestinal tract and lung (2). Both CHIA and CHIT1 are secreted as 50 kDa proteins. In contrast to CHIA, CHIT1 is not stable under acidic pHs and can be processed into a C-terminally truncated 39 kDa form (2, 3). CHIT1 is the best biomarker in the monitoring of Gaucher disease among the three most commonly used markers that also include acid phosphatase and angiotensin-converting enzyme (ACE) (4). CHIT1 is also a specific marker of macrophage activation in acute ischemic stroke (5).
References
- Aguilera, B. et al. (2003) J. Biol. Chem. 278:40911.
- Boot, R.G. et al. (2001) J. Biol. Chem. 276:6770.
- Renkema, G.H. et al. (1997) Eur. J. Biochem. 244:279.
- Vellodi, A. et al. (2005) J. Inherit. Metab. Dis. 28:585.
- Sotgiu, S. et al. (2005) Eur. Neurol. 54:149.
Alternate Names
CHI3, CHIT1
Entrez Gene IDs
1118 (Human)
Gene Symbol
CHIT1
UniProt
Additional Chitotriosidase/CHIT1 Products
Product Documents for Human Chitotriosidase/CHIT1 Antibody
Product Specific Notices for Human Chitotriosidase/CHIT1 Antibody
For research use only
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