Human Chorionic Gonadotropin alpha Chain ( alpha HCG) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4169
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 381012
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Chorionic Gonadotropin, alpha Chain ( alpha HCG)
Ala25-Ser116
Accession # P01215
Ala25-Ser116
Accession # P01215
Specificity
Detects human Chorionic Gonadotropin, alpha Chain ( alpha HCG) in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human FSH beta or recombinant rat FSH beta is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Chorionic Gonadotropin alpha Chain ( alpha HCG) Antibody
Chorionic Gonadotropin, alpha Chain ( alpha HCG) in Human Placenta.
Chorionic Gonadotropin, a Chain (a HCG) was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human Chorionic Gonadotropin, a Chain (a HCG) Monoclonal Antibody (Catalog # MAB4169) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Chorionic Gonadotropin alpha Chain ( alpha HCG) Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Sample: Immersion fixed paraffin-embedded sections of human placenta subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Western Blot
1 µg/mL
Sample: Recombinant Human Chorionic Gonadotropin, alpha Chain ( alpha HCG)
Sample: Recombinant Human Chorionic Gonadotropin, alpha Chain ( alpha HCG)
Reviewed Applications
Read 2 reviews rated 4.5 using MAB4169 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Chorionic Gonadotropin alpha Chain (HCG alpha)
Human Chorionic Gonadotropin, alpha chain (CGA) is a 92 aa glycopeptide that functions as the shared alpha subunit of the heterodimeric peptide hormones choriogonadotropin, leutinizing hormone, thryoid stimulating hormone, and follicle stimulating hormone. CGA circulates as a free molecule and in noncovalent complexes with the unique beta subunits of those hormones. CGA is secreted by the pituitary and placenta. Mature human CGA shares 69%‑73% aa sequence identity with canine, equine, feline, mouse, porcine, and rat CGA.
Alternate Names
alpha HCG, CGA, FSHA, GPHa1, LHA, TSHA
Entrez Gene IDs
1081 (Human)
Gene Symbol
CGA
UniProt
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Product Documents for Human Chorionic Gonadotropin alpha Chain ( alpha HCG) Antibody
Product Specific Notices for Human Chorionic Gonadotropin alpha Chain ( alpha HCG) Antibody
For research use only
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