HEK293 human embryonic kidney cell line transfected with either (A) human CHRM2 or (B) irrelevant protein, and eGFP, was stained with Mouse anti-human CHRM2 monoclonal antibody (Catalog # MAB6377) followed by Allophycocyanin-conjugated anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB002, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Applications for Human CHRM2 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line transfected with Human CHRM2 and eGFP

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human heart

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CHRM2

Muscarinic acetylcholine receptor M2 (CHRM2) is a 60 kDa 7-transmembrane glycoprotein that is predominantly expressed in the heart and central nervous system. CHRM2 mediates signal transduction in the autonomic nervous system in response to muscarinic agonists. Polymorphisms of CHRM2 are associated with alcohol dependence, depression, bipolar disorder, and recovery of heart function after myocardial infarction. Human CHRM2 shares 96% aa sequence identity with mouse and rat CHRM2.

Long Name

Cholinergic Receptor Muscarinic 2

Alternate Names

HM2, mAChR M2

Entrez Gene IDs

1129 (Human)

Gene Symbol

CHRM2

UniProt

P08172

Additional CHRM2 Products

Product Documents for Human CHRM2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CHRM2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CHRM2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6377

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all CHRM2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CHRM2 Antibody

Detection of CHRM2 in HEK293 Human Cell Line transfected with Human CHRM2 and eGFP by Flow Cytometry