Human Claudin-15 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10344
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 540315
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human Claudin-15
Accession # P56746
Accession # P56746
Specificity
Detects human Claudin-15 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Claudin-15 Antibody
Detection of Claudin-15 in HEK293 Human Cell Line Transfected with Human Claudin-15 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either (A) human Claudin-15 or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human Claudin-15 Monoclonal Antibody (Catalog # MAB10344) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.Applications for Human Claudin-15 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human Claudin-15 and eGFP
Sample: HEK293 Human Cell Line Transfected with Human Claudin-15 and eGFP
Reviewed Applications
Read 1 review rated 5 using MAB10344 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Claudin-15
CLDN15 is a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions.
Alternate Names
Claudin15, CLDN15
Gene Symbol
CLDN15
UniProt
Additional Claudin-15 Products
Product Documents for Human Claudin-15 Antibody
Product Specific Notices for Human Claudin-15 Antibody
For research use only
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