U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human CLEC-2A Monoclonal Antibody (Catalog # MAB7219, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Applications for Human CLEC-2A Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: U937 human histiocytic lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC-2A

CLEC-2A (C-type lectin domain family 2 member A), also known as PILAR and KACL, is an approximately 35 kDa type II transmembrane glycoprotein in the C-type lectin superfamily. It contains one C-type lectin domain in its extracellular region. Alternate splicing of human CLEC-2A generates an additional isoform with a substitution of 37 amino acids (aa) at the C-terminus. CLEC-2A is upregulated on antigen-activated CD8⁺ T cells and enhances T cell proliferation through interactions with CD161. It is also expressed on keratinocytes and binds to NKp65/KLRF2, triggering NK cell activation.

Long Name

C-type Lectin Domain Family 2, Member A

Alternate Names

CLEC2A, KACL, PILAR

Entrez Gene IDs

387836 (Human)

Gene Symbol

CLEC2A

UniProt

Q6UVW9 (Human)

Additional CLEC-2A Products

Product Documents for Human CLEC-2A Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CLEC-2A Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CLEC-2A Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7219

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all CLEC-2A Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CLEC-2A Antibody

Detection of CLEC-2A in U937 Human Cell Line by Flow Cytometry.