Human CNP Antibody

Name: Human CNP Antibody
Brand: R&D Systems
SKU: AF3127

R&D Systems, part of Bio-Techne | Catalog # AF3127

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all CNP Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CNP
Lys24-Cys126
Accession # P23582

Specificity

Detects human CNP in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human CNP Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human CNP, see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human CNP

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CNP

C-type natriuretic peptide (CNP) belongs to the natriuretic peptide family and functions in an autocrine or paracrine fashion. CNP interacts with the GC-B/NPR-B receptor to promote vasorelaxation, vascular remodeling, and the growth and differentiation of bone and neural tissue. CNP is synthesized as a prohormone that is cleaved intracellularly by furin, yielding a 49 aa propeptide and a 53 aa mature peptide. Additional cleavage of the mature peptide generates 29 aa and 22 aa peptides which have comparable activity in some assays. Within the 103 aa pro CNP, human shares 91% aa sequence identity with mouse and rat pro CNP.

Long Name

C-type Natriuretic Peptide

Alternate Names

NPPC

Entrez Gene IDs

4880 (Human)

Gene Symbol