Human Common gamma Chain/IL-2 R gamma Antibody
R&D Systems, part of Bio-Techne | Catalog # AF284
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry, Neutralization, Western Blot
Cited:
Flow Cytometry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Common gamma Chain/IL‑2 R gamma
Leu23-Asn254
Accession # P31785
Leu23-Asn254
Accession # P31785
Specificity
Detects human Common gamma Chain/IL‑2 R gamma in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse IL-2 R gamma is observed, less than 10% cross-reactivity with recombinant human (rh) IL-2 R beta is observed, and less than 2% cross-reactivity with rhIL-2 R alpha is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<1.0 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human Common gamma Chain/IL-2 R gamma Antibody
Cell Proliferation Induced by IL‑2 and Neutralization by Human Common gamma Chain/ IL‑2 R gamma Antibody.
Recombinant Human IL-2 (Catalog # 202-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Common gamma Chain/ IL-2 R gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF284). The ND50 is typically 20-60 µg/mL.Applications for Human Common gamma Chain/IL-2 R gamma Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Daudi human Burkitt's lymphoma cell line
Sample: Daudi human Burkitt's lymphoma cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human Common gamma Chain/IL-2 R gamma (Catalog # 384-RG)
Sample: Recombinant Human Common gamma Chain/IL-2 R gamma (Catalog # 384-RG)
Neutralization
Measured by its ability to neutralize IL‑2-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND50) is typically 20-60 µg/mL in the presence of 30 ng/mL Recombinant Human IL‑2.
Reviewed Applications
Read 1 review rated 5 using AF284 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Common gamma Chain/IL-2 R gamma
The gamma chain of the high affinity functional human IL-2 receptor complex belongs to the hematopoietin receptor family. IL-2 R gamma is a 369 amino acid residue protein consisting of a 22 residue signal sequence, a 232 residue extracellular domain, a 29 residue transmembrane domain and an 86 residue cytoplasmic domain. Although IL-2 R gamma by itself does not bind IL-2 with any appreciable affinity, it is required for IL-2 receptor signaling. Besides IL-2, the gamma chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. It has been proposed that IL-2 R gamma be designated the common gamma chain ( gammac). The site of molecular defects in X-linked SCID (severe combined immunodeficiency) has now been mapped to the IL-2 R gamma gene.
References
- Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
- Noguchi, M. et al. (1993) Science 262:1877.
Long Name
IL-2 Receptor gamma
Alternate Names
CD132, IL-2 R gamma, IL2R gamma, IL2RG
Gene Symbol
IL2RG
UniProt
Additional Common gamma Chain/IL-2 R gamma Products
Product Specific Notices for Human Common gamma Chain/IL-2 R gamma Antibody
For research use only
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