Human Complement Component C1qA Antibody

Name: Human Complement Component C1qA Antibody
Brand: R&D Systems
SKU: MAB4035

R&D Systems, part of Bio-Techne | Catalog # MAB4035

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG_2B Clone # 394107

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Product Summary for Human Complement Component C1qA Antibody

Immunogen

E. coli-derived recombinant human Complement Component C1qA
Lys110-Ala245
Accession # P02745

Specificity

Detects recombinant human Complement Component C1qA in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Complement Component C1qB is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Scientific Data Images for Human Complement Component C1qA Antibody

Detection of Complement Component C1qA in Human Placenta.

Complement Component C1qA was detected in immersion fixed paraffin-embedded sections of Human Placenta using Rat Anti-Human Complement Component C1qA Monoclonal Antibody (Catalog # MAB4035) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in cytotrophoblast in chorionic villi. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Complement Component C1qA Antibody

Application

Recommended Usage

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Placenta

Western Blot

1 µg/mL
Sample: Recombinant Human Complement Component C1qA

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Component C1qA

C1qA, one of three subunits of complement component C1q, contains a collagen-like domain and a globular C1q domain. Within the globular domain, human C1qA shares 73% and 77% aa sequence identity with mouse and rat C1qA, respectively. Six disulfide-linked heterodimers of C1qA and C1qB associate with three disulfide-linked homodimers of C1qC to form C1q. Deficiency in C1qA is associated with the development of autoimmunity.

Long Name

Complement Component 1q Subcomponent, A Chain

Alternate Names

C1QA

Entrez Gene IDs

712 (Human); 12259 (Mouse); 298566 (Rat)

Gene Symbol