Human Complement Component C2b Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1936
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 269716
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Complement Component C2
Ala21-Leu752
Accession # P06681
Ala21-Leu752
Accession # P06681
Specificity
Detects human Complement Component C2b in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with recombinant human (rh) C1r, rhC1s, rhC3r, rhC5a, rhFactor B, or rhFactor I is observed. In Western blots, this antibody recognizes rhC2 and rhC2B.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications for Human Complement Component C2b Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Complement Component C2b, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Complement Component C2b, see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human Complement Component C2b
under non-reducing conditions only
Sample: Recombinant Human Complement Component C2b
under non-reducing conditions only
Reviewed Applications
Read 4 reviews rated 4.3 using MAB1936 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Complement Component C2b
C2 is a key protease involved in the classical pathway of complement. It is synthesized as a single-chain and is proteolytically processed into two chains, C2a (aa 244‑752) and C2b (aa 21‑243). C2a consists of a vWF domain and a serine protease domain. C2b contains 3 Sushi (SCR) domains.
Alternate Names
ARMD14, CO2
Gene Symbol
C2
UniProt
Additional Complement Component C2b Products
Product Documents for Human Complement Component C2b Antibody
Product Specific Notices for Human Complement Component C2b Antibody
For research use only
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