Human Complement Factor H Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4779
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 556317
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Complement Factor H
Ser860-Arg1231
Accession # P08603
Ser860-Arg1231
Accession # P08603
Specificity
Detects human Complement Factor H in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Complement Factor H Antibody
Detection of Human Complement Factor H by Western Blot.
Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 2 µg/mL of Human Complement Factor H Monoclonal Antibody (Catalog # MAB4779) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Complement Factor H at approximately 155 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Complement Factor H Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Complement Factor H, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Complement Factor H, see our available Western blot detection antibodies
Western Blot
2 µg/mL
Sample: Human liver tissue
Sample: Human liver tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Complement Factor H
Complement Factor H (CFH) is a 155 kDa glycoprotein that negatively regulates the alternative pathway complement cascade. It is secreted by Kupffer cells, hepatocytes, vascular endothelial cells, and platelets and circulates in the serum at high concentration. CFH interacts with cell surface polyanions and prevents local complement activation by sequestering complement component C3b, accelerating the decay of C3 and C5, and functioning as a cofactor for Factor I. CFH is composed of 20 SCR (short consensus repeats). Alternate splicing generates an isoform that is truncated following SCR7. Within SCR5-20 (aa 260-1231), human CFH shares 60% and 62% aa sequence identity with mouse and rat CFH, respectively.
Alternate Names
ARMD4, ARMS1, beta-1-H-globulin, CFH, CFHL3, FH, FHL1, HF1, HF2, HUS
Gene Symbol
CFH
UniProt
Additional Complement Factor H Products
Product Documents for Human Complement Factor H Antibody
Product Specific Notices for Human Complement Factor H Antibody
For research use only
Loading...
Loading...
Loading...
Loading...