Human Corin Antibody

Detection of Human Corin by Western Blot.

Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Corin Antigen Affinity-purified Polyclonal Antibody (Catalog # af2209) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Corin at approximately 85 and 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Corin in Human Liver.

Corin was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Corin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2209) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in canaliculi. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Corin in Human Heart.

Corin was detected in immersion fixed paraffin-embedded sections of human heart using Goat Anti-Human Corin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2209) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.