Human Corneodesmosin Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5725

R&D Systems, part of Bio-Techne
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AF5725
AF5725-SP
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Biotin
BAF5725
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Corneodesmosin Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Corneodesmosin
Lys33-Pro529 (Ser153 del)
Accession # NP_001255

Specificity

Detects human Corneodesmosin in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Corneodesmosin Antibody

Corneodesmosin antibody in Human Skin by Immunohistochemistry (IHC-P).

Corneodesmosin in Human Skin.

Corneodesmosin was detected in immersion fixed paraffin-embedded sections of human skin using Sheep Anti-Human Corneodesmosin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5725) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Corneodesmosin Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Corneodesmosin

Corneodesmosin, also known as CDSN and the S gene product, is a highly polymorphic secreted glycoprotein that plays an important structural role in the skin (1). It is expressed by differentiated keratinocytes in the corneal layer of the skin and is a major component of corneodesmosomes (2-4). It is also expressed in the inner root sheath of hair follicles (5, 6). Corneodesmosome has a high content of glycine, serine, and proline residues that promote its folding into a series of Gly-loop domains (2, 7). Corneodesmosin forms oligomers and associates homophilically to strengthen the adhesion between corneocytes (8, 9). Corneodesmosin-deficient mice exhibit a detachment of the corneal layer of the skin as well as hypotrichosis of the scalp and baldness (6, 10). Corneodesmosin is secreted by keratinocytes as a 52-56 kDa molecule which is then subjected to repeated sequential N- and C-terminal proteolysis (11). Species of 46, 43, 36, and 15 kDa are present in corneocytes (7, 11). Cleavage of the N-terminal Gly-loop diminishes Corneodesmosin’s ability to mediate adhesion, and this is a prerequisite for normal desquamation of the skin (8, 9). Reduced proteolysis of Corneodesmosin in psoriasis lesions is associated with the persistence of corneodesmosomes and scale retention (12). Premature truncation of Corneodesmosin is associated with hypotrichosis of the scalp (13).

References

  1. Pierard, G.E. et al. (2000) Int. J. Mol. Med. 6:217.
  2. Zhou, Y. and D.D. Chaplin (1993) Proc. Natl. Acad. Sci. USA 90:9470.
  3. Haftek, M. et al. (1997) Br. J. Dermatol. 137:864.
  4. Simon, M. et al. (1997) J. Biol. Chem. 272:31770.
  5. Mils, V. et al. (1992) J. Histochem. Cytochem. 40:1329.
  6. Matsumoto, M. et al. (2008) Proc. Natl. Acad. Sci. USA 105:6720.
  7. Guerrin, M. et al. (1998) J. Biol. Chem. 273:22640.
  8. Jonca, N. et al. (2002) J. Biol. Chem. 277:5024.
  9. Caubet, C. et al. (2004) J. Invest. Dermatol. 122:747.
  10. Leclerc, E.A. et al. (2009) J. Cell Sci. 122:2699.
  11. Simon, M. et al. (2001) J. Biol. Chem. 276:20292.
  12. Simon, M. et al. (2008) Br. J. Dermatol. 159:77.
  13. Levy-Nissenbaum, E. et al. (2003) Nat. Genet. 34:151.

Alternate Names

CDSN, D6S586E, HTSS

Entrez Gene IDs

1041 (Human); 386463 (Mouse)

Gene Symbol

CDSN

UniProt

NP_001255

Additional Corneodesmosin Products

Product Documents for Human Corneodesmosin Antibody

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Product Specific Notices for Human Corneodesmosin Antibody

For research use only

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