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Human CRABP2 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5740

R&D Systems, part of Bio-Techne
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AF5740
AF5740-SP

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

E. coli-derived recombinant human CRABP2
Met1-Glu138
Accession # P29373

Specificity

Detects endogenous human CRABP2 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human CRABP2 Antibody

Detection of Human CRABP2 antibody by Western Blot.

Detection of Human CRABP2 by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line, MDA-MB-468 human breast cancer cell line, and T47D human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Human CRABP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5740) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CRABP2 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human CRABP2 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: MCF-7 human breast cancer cell line, MDA-MB-468 human breast cancer cell line, and T47D human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRABP2

CRABP2 (Cellular retinoic acid binding protein type II; also RABP2) is a 14 kDa cytosolic member of the fatty acid binding protein family of molecules. It is expressed by a variety of cell types, including keratinocytes, corneal epithelium, fibroblasts and visceral smooth muscle. CRABP2 binds bioactive retinoic acid and transports it to the nucleus, where, in contrast to CRABP1, it facilitates its transfer to the retinoic acid receptor. Human CRABP2 is 138 amino acids (aa) in length and contains one NLS (aa 21‑31) and a series of beta-strands forming a beta-barrel that accommodates retinoic acid in its interior (aa 41‑135).

Long Name

Cellular Retinoic Acid Binding Protein 2

Alternate Names

CRABP-II, RBP6

Entrez Gene IDs

1382 (Human); 12904 (Mouse); 29563 (Rat)

Gene Symbol

CRABP2

UniProt

Additional CRABP2 Products

Product Documents for Human CRABP2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CRABP2 Antibody

For research use only

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