Human CRABP2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5740
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CRABP2
Met1-Glu138
Accession # P29373
Met1-Glu138
Accession # P29373
Specificity
Detects endogenous human CRABP2 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human CRABP2 Antibody
Detection of Human CRABP2 by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line, MDA-MB-468 human breast cancer cell line, and T47D human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Human CRABP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5740) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CRABP2 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human CRABP2 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: MCF-7 human breast cancer cell line, MDA-MB-468 human breast cancer cell line, and T47D human breast cancer cell line
Sample: MCF-7 human breast cancer cell line, MDA-MB-468 human breast cancer cell line, and T47D human breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CRABP2
Long Name
Cellular Retinoic Acid Binding Protein 2
Alternate Names
CRABP-II, RBP6
Gene Symbol
CRABP2
UniProt
Additional CRABP2 Products
Product Documents for Human CRABP2 Antibody
Product Specific Notices for Human CRABP2 Antibody
For research use only
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