Human Creatine Kinase MM/CKMM Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5564
Key Product Details
Species Reactivity
Human
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 492731
Product Specifications
Immunogen
Human cardiac muscle-derived Creatine Kinase, Muscle/CKMM
Specificity
Detects human Creatine Kinase, Muscle/CKMM in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Creatine Kinase MM/CKMM Antibody
Detection of Human Creatine Kinase MM/CKMM by Western Blot.
Western blot shows lysate of human heart tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Creatine Kinase MM/ CKMM Monoclonal Antibody (Catalog # MAB5564) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Creatine Kinase MM/CKMM at approximately 40-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Creatine Kinase MM/CKMM by Simple WesternTM.
Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Creatine Kinase MM/ CKMM at approximately 51 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human Creatine Kinase MM/CKMM Monoclonal Antibody (Catalog # MAB5564). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human Creatine Kinase MM/CKMM Antibody
Application
Recommended Usage
Simple Western
1 µg/mL
Sample: Human heart tissue
Sample: Human heart tissue
Western Blot
0.1 µg/mL
Sample: Human heart tissue
Sample: Human heart tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Creatine Kinase MM/CKMM
Creatine kinase (CK) is a 40 kDa cytoplasmic enzyme that is integral to energy metabolism in striated muscle through the maintenance of ATP levels. It is a dimeric protein composed of tissue specific M (muscle) and B (brain) subunits. The homodimeric CKMM isozyme is predominantly expressed in differentiated skeletal and cardiac muscle fibers. The M subunit of human creatine kinase shares 97% aa sequence identity with the mouse and rat M subunit and 81% with the human B subunit.
Long Name
Creatine Kinase, Muscle
Alternate Names
CKM, CKMM, M-CK
Gene Symbol
CKM
Additional Creatine Kinase MM/CKMM Products
Product Documents for Human Creatine Kinase MM/CKMM Antibody
Product Specific Notices for Human Creatine Kinase MM/CKMM Antibody
For research use only
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