Human Creatine Kinase MM/CKMM Antibody
R&D Systems, part of Bio-Techne | Catalog # G-113-C
Key Product Details
Species Reactivity
Human
Applications
Simple Western
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Human cardiac muscle-derived Creatine Kinase, Muscle/CKMM
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Creatine Kinase MM/CKMM Antibody
Detection of Human Creatine Kinase MM/CKMM by Simple WesternTM.
Simple Western lane view shows lysates of Human Heart, loaded at 0.2 mg/mL. A specific band was detected for Creatine Kinase MM/CKMM at approximately 50 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Creatine Kinase MM/CKMM Antigen Affinity-purified Polyclonal Antibody (Catalog # G-113-C). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.Applications for Human Creatine Kinase MM/CKMM Antibody
Application
Recommended Usage
Simple Western
5 µg/mL
Sample: Human Heart
Sample: Human Heart
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute with sterile PBS. If 0.5 mL of PBS is used, the antibody concentration will be 0.2 mg/mL.
Formulation
Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and Sodium Azide with Trehalose.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Creatine Kinase MM/CKMM
Creatine kinase (CK) is a 40 kDa cytoplasmic enzyme that is integral to energy metabolism in striated muscle through the maintenance of ATP levels. It is a dimeric protein composed of tissue specific M (muscle) and B (brain) subunits. The homodimeric CKMM isozyme is predominantly expressed in differentiated skeletal and cardiac muscle fibers. The M subunit of human creatine kinase shares 97% aa sequence identity with the mouse and rat M subunit and 81% with the human B subunit.
Long Name
Creatine Kinase, Muscle
Alternate Names
CKM, CKMM, M-CK
Gene Symbol
CKM
Additional Creatine Kinase MM/CKMM Products
Product Documents for Human Creatine Kinase MM/CKMM Antibody
Product Specific Notices for Human Creatine Kinase MM/CKMM Antibody
For research use only
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