Human CTRP3/C1qTNF3/CORS26 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB79251

R&D Systems, part of Bio-Techne
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MAB79251-100
MAB79251-SP
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Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # 860101

View all CTRP3/C1qTNF3/CORS26 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line, NS0-derived human CTRP3/C1qTNF3/CORS26
Gln23-Lys246
Accession # Q9BXJ4-1

Specificity

Detects human CTRP3/C1qTNF3/CORS26 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Scientific Data Images for Human CTRP3/C1qTNF3/CORS26 Antibody

Human CTRP3/C1qTNF3/CORS26 Antibody in ELISA Standard Curve.

Human CTRP3/C1qTNF3/CORS26 ELISA Standard Curve.

Recombinant Human CTRP3/C1qTNF3/CORS26 protein was serially diluted 2-fold and captured by Mouse Anti-Human CTRP3/C1qTNF3/CORS26 Monoclonal Antibody (MAB7925) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human CTRP3/C1qTNF3/CORS26 Monoclonal Antibody (Catalog # MAB79251) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Applications for Human CTRP3/C1qTNF3/CORS26 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human CTRP3/C1qTNF3/CORS26 Monoclonal Antibody(Catalog # MAB7925).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human CTRP3/C1qTNF3/CORS26 DuoSet ELISA Kit (Catalog # DY7925-05) for convenient development of a sandwich ELISA.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CTRP3/C1qTNF3/CORS26

CTRP3 (Complement C1q TNF-related Protein 3/C1qTNF3) also known as CORS-26 (Collagenous Repeat-containing Sequence of 26 kDa protein), Cartonectin and Cartducin, is a 30-32 kDa, secreted member of the C1q and TNF-related (CTRP) superfamily of molecules (1). The mature protein is 224 aa in length. It contains an N-terminal collagen-like domain followed by a C-terminal globular region. Human CTRP3 shares 99% aa sequence identity with the mouse CTRP3 (2). Like other CTRP members, CTRP3 has a trimeric structure and can assemble into hexameric or higher order molecular forms (3). It is expressed by a variety of cells, including adipocytes, cartilage, fibroblasts, monocytes and proliferating chondrocytes (4). The inflammatory effects of LPS, TLR-4 and fatty acids have been shown to be inhibited by CTRP3 in adipocytes and monocytes (5). In mouse models, CTRP3 has been shown to lower glucose levels and decrease gluconeogenic gene expression (6). Inhibition of 3T3-L1 pre-adipocyte differentiation to adipocytes is associated with CTRP3 treatment, demonstrating potential anti-obesity effects (7). Treatment with CTRP3 results in the proliferation of skeletal muscle C2C12 cells and inhibition of C2C12 myotube differentiation, mediated by the ERK pathway (8). Due to the variety of functions in metabolism and inflammation, CTRP3 is a potential new target of type 2 diabetes treatment.

References

  1. Wong, G.W. et al. (2004) Proc. Natl. Acad. Sci. U.S.A. 101:10302.
  2. Schaffler A. et al. (2003). Biochim Biophys Acta.1630:123.
  3. Suzuki S. et al. (2007) FEBS Lett. 581: 809.
  4. Weigert J. et al. (2005) FEBS Lett. 579: 5565.
  5. Kopp, A. et al. (2010) Endocrinology. 151:5267.
  6. Peterson J. M. et al. (2010) J Biol Chem. 285:39691.
  7. Nishimoto, H. et al. (2017) Cell Bio Int. 41:197.
  8. Otani M. et al. (2015) Mol Cell Biochem. 409:271.

Long Name

C1q and Tumor Necrosis Factor-related Protein 3

Alternate Names

C1qTNF3, Cartducin, CORS26

Entrez Gene IDs

114899 (Human); 81799 (Mouse)

Gene Symbol

C1QTNF3

UniProt

Q9BXJ4-1

Additional CTRP3/C1qTNF3/CORS26 Products

Product Documents for Human CTRP3/C1qTNF3/CORS26 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CTRP3/C1qTNF3/CORS26 Antibody

For research use only

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