Detects human CTRP5/C1qTNF5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CTRP1 or rhCTRP4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human CTRP5/C1qTNF5 Antibody

CTRP5/C1qTNF5 in Human Liver.

CTRP5/C1qTNF5 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human CTRP5/C1qTNF5 Monoclonal Antibody (Catalog # MAB3167) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to sinusoids. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of CTRP5/C1qTNF5 in A172 Human Cell Line by Flow Cytometry.

A172 human glioblastoma cell line was stained with Mouse Anti-Human CTRP5/C1qTNF5 Monoclonal Antibody (Catalog # MAB3167, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human CTRP5/C1qTNF5 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: A172 human glioblastoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Western Blot

1 µg/mL
Sample: Recombinant Human CTRP5/C1qTNF5

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CTRP5/C1qTNF5

CTRP5, also known as C1qTNF5, belongs to the highly conserved family of Acrp30/Adiponectin paralogs known as C1q and TNF‑related protein family. All family members share a modular organization comprising an N‑terminal signal peptide, a short variable region, a collagenous domain and a C‑terminal globular domain. CTRP proteins are predicted to have trimeric structures that assemble into hexameric and higher order molecular forms. Human and mouse CTRP5 share 94% amino acid sequence identity.

Long Name

C1q And Tumor Necrosis Factor Related Protein 5

Alternate Names

C1qTNF5

Entrez Gene IDs

114902 (Human); 235312 (Mouse)

Gene Symbol

C1QTNF5

UniProt

Q9BXJ0

Additional CTRP5/C1qTNF5 Products

Product Documents for Human CTRP5/C1qTNF5 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CTRP5/C1qTNF5 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CTRP5/C1qTNF5 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3167

Key Product Details

Species Reactivity

Validated:

Cited:

Applications