Human Cyclin E2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7444
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 718916
Product Specifications
Immunogen
E. coli-derived recombinant human Cyclin E2
Met1-Gln140
Accession # O96020
Met1-Gln140
Accession # O96020
Specificity
Detects human Cyclin E2 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Cyclin E2 Antibody
Cyclin E2 in Human Breast.
Cyclin E2 was detected in immersion fixed paraffin-embedded sections of human breast using Mouse Anti-Human Cyclin E2 Monoclonal Antibody (Catalog # MAB7444) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Cyclin E2 in Human Cervical Cancer Tissue.
Cyclin E2 was detected in immersion fixed paraffin-embedded sections of human cervical cancer tissue using Mouse Anti-Human Cyclin E2 Monoclonal Antibody (Catalog # MAB7444) at 1.2 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Cyclin E2 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast and human cervical cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human breast and human cervical cancer tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cyclin E2
Alternate Names
CCNE2, CYCE2
Gene Symbol
CCNE2
UniProt
Additional Cyclin E2 Products
Product Documents for Human Cyclin E2 Antibody
Product Specific Notices for Human Cyclin E2 Antibody
For research use only
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