Human Cystatin A Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1407
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Cystatin A
Ile2-Phe98
Accession # P01040
Ile2-Phe98
Accession # P01040
Specificity
Detects Cystatin A in direct ELISAs and Western blots. In Western blots, less than 1% cross‑reactivity with recombinant mouse (rm) Cystatin A, recombinant human (rh) Cystatin B, rhCystatin C, rhCystatin D, rhCystatin E/M, rhCystatin F, rhCystatin S, rhCystatin SA and rhCystatin SN is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Cystatin A Antibody
Detection of Human Cystatin A by Western Blot.
Western Blot shows lysates of THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 2 µg/ml of Goat Anti-Human Cystatin A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1407) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cystatin A at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.Applications for Human Cystatin A Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Cystatin A (Catalog # 1407-PI), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Cystatin A (Catalog # 1407-PI), see our available Western blot detection antibodies
Western Blot
2 µg/mL
Sample: THP-1 human acute monocytic leukemia cell line
Sample: THP-1 human acute monocytic leukemia cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cystatin A
Cystatin A, also called stefin A, Cystatin AS or keratolinin, is a member of family 1 of the cystatin superfamily (1, 2). Like Cystatin B, it is an intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as cathepsins B, H and L (3). For example, immunohistochemical analysis of Cystatin A and cathepsin L is a useful indicator for malignancy in human epidermal keratinocytes (4). The ratio of cathepsin B and Cystatin A can be used in the differential diagnosis and treatment of patients with prostate carcinoma (5). The human Cystatin A consists of 98 amino acids (1).
References
- Kartasova, T. et al. (1987) Nucleic Acids Res. 15:5945.
- Abrahamson, M. (1994) Methods Enzymol. 244:685.
- Jenko, S. et al. (2003) J. Mol. Biol. 326:875.
- Palungwachira, P. et al. (2002) J. Dermatol. 29:573.
- Sinha, A.A. et al. (2002) Cancer 94:3141.
Alternate Names
AREI, CSTA, Stefin A, STF1, STFA
Entrez Gene IDs
1475 (Human)
Gene Symbol
CSTA
UniProt
Additional Cystatin A Products
Product Documents for Human Cystatin A Antibody
Product Specific Notices for Human Cystatin A Antibody
For research use only
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