Human Cystatin SN Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1285
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
ELISA Development, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 213139
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cystatin SN
Trp21-Ser141 (predicted)
Accession # P01037
Trp21-Ser141 (predicted)
Accession # P01037
Specificity
Detects human Cystatin SN in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) Cystatins A, B, C, D, S, SA, E/M, rhFetuin A, rhFetuin B, rhHPRG, or rhThrombin is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human Cystatin SN Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Cystatin SN (Catalog # 1285-PI), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Cystatin SN (Catalog # 1285-PI), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human Cystatin SN (Catalog # 1285-PI)
Sample: Recombinant Human Cystatin SN (Catalog # 1285-PI)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cystatin SN
Cystatin SN is a member of family 2 of the cystatin superfamily (1). Together with cystatins S and SA, it is produced by the salivary gland and secreted largely in the submandibular/sublingual saliva (2). Cystatin SN inhibits members of the papain family including cathepsins B, C, H and L (3).
References
- Abrahamson, M. (1994) Methods Enzymol. 244:685.
- Baron, A.C. et al. (1999) Oral Dis. 5:344.
- Baron, A. et al. (1999) Oral Dis. 5:234.
Alternate Names
CST1
Entrez Gene IDs
1469 (Human)
Gene Symbol
CST1
UniProt
Additional Cystatin SN Products
Product Documents for Human Cystatin SN Antibody
Product Specific Notices for Human Cystatin SN Antibody
For research use only
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