I notice you sell both antibodies and recombinant protein for human cystatin SN. Could you perhaps develop an ELISA for this protein?

NBP1-55995 and H00001469-B01P are both suitable for use as antibodies in ELISA. While a kit is not in development, you could use these to make your own ELISA kit, as many of our users do.

Human Cystatin SN Antibody

R&D Systems, part of Bio-Techne | Catalog # AF1285

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Citations (2)

Product Datasheet / COA / SDS

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Conjugate

Catalog #

Biotin

BAF1285

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Cystatin SN Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Cystatin SN
Trp21-Ser141
Accession # P01037

Specificity

Detects human Cystatin SN in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant human (rh) Cystatin SA is observed, 15% cross-reactivity with rhCystatin S is observed, 5% cross-reactivity with rhCystatin C is observed, and less than 1% cross-reactivity with rhCystatin A, rhCystatin B, rhCystatin E/M, and rhCystatin F is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Cystatin SN Antibody

Application

Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Cystatin SN (Catalog # 1285-PI), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human Cystatin SN (Catalog # 1285-PI)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cystatin SN

Cystatin SN is a member of family 2 of the cystatin superfamily (1). Together with cystatins S and SA, it is produced by the salivary gland and secreted largely in the submandibular/sublingual saliva (2). Cystatin SN inhibits members of the papain family including cathepsins B, C, H and L (3).

References

Abrahamson, M. (1994) Methods Enzymol. 244:685.
Baron, A.C. et al. (1999) Oral Dis. 5:344.
Baron, A. et al. (1999) Oral Dis. 5:234.

Alternate Names

CST1

Entrez Gene IDs

1469 (Human)

Gene Symbol