Human DARC APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB4139A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 358307
Product Specifications
Immunogen
HEK293 human embryonic kidney cell line transfected with human DARC
Met1-Ser336
Accession # Q16570
Met1-Ser336
Accession # Q16570
Specificity
Detects human DARC. Stains human DARC transfected cells but not irrelevant transfectants.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human DARC APC-conjugated Antibody
Detection of DARC in Human Red Blood Cells by Flow Cytometry.
Human red blood cells were stained with Mouse Anti-Human DARC APC-conjugated Monoclonal Antibody (Catalog # FAB4139A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human DARC APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Human red blood cells
Sample: Human red blood cells
Reviewed Applications
Read 1 review rated 4 using FAB4139A in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: DARC
Duffy Antigen Receptor for Chemokines (DARC), also known as Fy glycoprotein and CD234, is a 7TM non-signaling chemokine receptor that is expressed on venular endothelial cells and erythrocytes. DARC binds and mediates the internalization of several inflammatory CC and CXC chemokines. It transports chemokines to the lumenal face of venular endothelium to promote the extravasation of leukocytes to sites of inflammation. DARC polymorphisms are important in blood transfusion compatibility and determine the susceptibility of erythrocytes to Plasmodium vivax infection. Human DARC shares 60% amino acid sequence identity with mouse DARC.
Long Name
Duffy Antigen, Receptor For Chemokines
Alternate Names
ACKR1, CD234, Duffy Antigen, Fy Antigen, GPD, GpFy
Gene Symbol
ACKR1
UniProt
Additional DARC Products
Product Documents for Human DARC APC-conjugated Antibody
Product Specific Notices for Human DARC APC-conjugated Antibody
For research use only
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