Human DC-LAMP Lumenal Domain Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4087
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Western Blot
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human DC‑LAMP Lumenal Domain
Asp21-Thr381
Accession # EAW78337
Asp21-Thr381
Accession # EAW78337
Specificity
Detects human DC‑LAMP Lumenal Domain in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human LAMP is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human DC-LAMP Lumenal Domain Antibody
Detection of DC-LAMP in Human Mature Dendritic Cells by Flow Cytometry.
Human mature dentritic cells differentated from human peripheral blood mononuclear cell derived CD14+cells treated with 20 ng/mL Recombinant Human IL-4 (204-IL) and 50 ng/mL Recombinant Human GM-CSF (215-GM) for 7 days and 1 µg/mL LPS, 20 ng/mL Recombinant Human TNF-a (210-TA), and 20 ng/mL Recombinant Human IL-1 beta/IL-1F2 (201-LB) for last 24 hours were stained with Goat Anti-Human DC-LAMP Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4087, filled histogram) or isotype control (AB-108-C, open histogram), followed by Donkey Anti-Goat IgG (H+L) Allophycocyanin (F0108).Applications for Human DC-LAMP Lumenal Domain Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human mature dentritic cells differentated from human peripheral blood mononuclear cell derived CD14+ cells treated with Recombinant Human IL‑4 (Catalog # 204-IL), Recombinant Human GM-CSF (Catalog # 215-GM), LPS, Recombinant Human TNF‑ alpha (Catalog # 210-TA), and Recombinant Human IL‑1 beta/IL‑1F2 (Catalog # 201-LB)
Sample: Human mature dentritic cells differentated from human peripheral blood mononuclear cell derived CD14+ cells treated with Recombinant Human IL‑4 (Catalog # 204-IL), Recombinant Human GM-CSF (Catalog # 215-GM), LPS, Recombinant Human TNF‑ alpha (Catalog # 210-TA), and Recombinant Human IL‑1 beta/IL‑1F2 (Catalog # 201-LB)
Western Blot
0.1 µg/mL
Sample: Recombinant Human DC-LAMP Lumenal Domain
Sample: Recombinant Human DC-LAMP Lumenal Domain
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DC-LAMP
DC-LAMP (dendritic cell lysosome-associated membrane protein: also known as CD208 and LAMP3) is a 50‑70 kDa member of the LAMP family of proteins. Mature human DC-LAMP is a 389 amino acid (aa) type I transmembrane protein. It has a 254 aa lumenal N‑terminus and a short 14 aa cytoplasmic tail. The molecule is found in type II pneumocytes, interdigitating DC, and various tumors. DC-LAMP serves as a marker of human mature DC, and it may play a role in normal lysosome and endosome function. Over the region used as immunogen, human DC-LAMP shares 72% and 55% aa sequence identity with canine and mouse DC-LAMP, respectively.
Long Name
Dendritic Cell-lysosome-associated Membrane Glycoprotein
Alternate Names
CD208, DCLAMP, LAMP3, TSC403
Gene Symbol
LAMP3
UniProt
Additional DC-LAMP Products
Product Documents for Human DC-LAMP Lumenal Domain Antibody
Product Specific Notices for Human DC-LAMP Lumenal Domain Antibody
For research use only
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