Human Desmoglein-4 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6589
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 683135
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Desmoglein-4
Glu24-Ala631
Accession # Q86SJ6
Glu24-Ala631
Accession # Q86SJ6
Specificity
Detects human Desmoglein-4 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity
with recombinant human Desmoglein-1, -2, or -3 is observed and approximately
40% cross-reactivity with recombinant mouse Desmoglein-4 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Desmoglein-4 Antibody
Detection of Human Desmoglein-4 by Western Blot.
Western blot shows lysates of human skin tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Desmoglein-4 Monoclonal Antibody (Catalog # MAB6589) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Desmoglein-4 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Desmoglein-4 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Human skin tissue
Sample: Human skin tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Desmoglein-4
Desmoglein-4 (DSG4) is a 110-130 kDa type I transmembrane glycoprotein in the cadherin family of calcium-dependent cell adhesion molecules. Desmogleins interact with transmembrane Desmocollins to form the adhesive components of desmosomes on epithelial cells. Desmoglein-4 contains four cadherin repeats in its extracellular region and two desmoglein repeat domains in its cytoplasmic region. Alternate splicing generates an additional isoform with a substitution in the cytoplasmic region. Desmoglein-4 is expressed on keratinocytes and trichocytes in the epidermis and hair follicles. It is required for hair follicle development, and DSG4 mutations are associated with autosomal recessive hypotrichosis. Within amino acids 24-631 (which includes the 26 aa propeptide and the ECD), human Desmoglein-4 shares 82% aa sequence identity with mouse and rat Desmoglein-4.
Alternate Names
Cad13, CDGF13, CDHF13, Desmoglein4, DSG4
Gene Symbol
DSG4
UniProt
Additional Desmoglein-4 Products
Product Documents for Human Desmoglein-4 Antibody
Product Specific Notices for Human Desmoglein-4 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...