Human DGK-delta Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7426

R&D Systems, part of Bio-Techne
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MAB7426
MAB7426-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 625423

View all DGK-delta Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DGF-delta
Ile570-Thr717
Accession # Q16760

Specificity

Detects human DGF-delta in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human DGK-delta Antibody

Detection of Human DGK-d antibody by Western Blot.

Detection of Human DGK‑ delta by Western Blot.

Western blot shows lysates of TT human medullary thyroid cancer cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human DGK-d Monoclonal Antibody (Catalog # MAB7426) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DGK-d at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human DGK-delta Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: TT human medullary thyroid cancer cell line and K562 human chronic myelogenous leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DGK-delta

Diacylglycerol kinase delta (DGK-delta) is a widely expressed 140 kDa member of the DGK family of kinases that phosphorylate the lipid diacylglycerol. DGK-delta contains a pleckstrin homology domain (aa 53-146), two zinc finger domains (aa 163-213 and aa 235-286), a catalytic domain (aa 317-451), and a sterile alpha motif (aa 1145‑1208). An isoform of DGK-delta with a shortened N-terminus is expressed predominantly in ovary and spleen. The two isoforms can form intracellular homooligomers or heterooligomers with each other. The oligomers are disrupted by PKC following phorbol ester stimulation. DGK-delta regulates PKC mediated signaling downstream of the EGF receptor. Within aa 570-717, human DGK-delta shares 82% aa sequence identity with mouse and rat DGK-delta.

Long Name

Diacylglycerol Kinase, delta 130 kDa

Alternate Names

DGKD, DGKdelta

Entrez Gene IDs

8527 (Human)

Gene Symbol

DGKD

UniProt

Q16760

Additional DGK-delta Products

Product Documents for Human DGK-delta Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human DGK-delta Antibody

For research use only

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