Human DGK- zeta Antibody

R&D Systems, part of Bio-Techne | Catalog # AF6099

R&D Systems, part of Bio-Techne
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AF6099
AF6099-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all DGK-zeta Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DGK-zeta
Gln800-Val945
Accession # Q13574

Specificity

Detects human DGK-zeta in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human DGK- zeta Antibody

Detection of Human DGK-? antibody by Western Blot.

Detection of Human DGK-zeta by Western Blot.

Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human DGK-zeta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6099) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for DGK-zeta at approximately 124 & 114 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human DGK- zeta Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Ramos human Burkitt's lymphoma cell line and K562 human chronic myelogenous leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DGK-zeta

DGK- zeta (Diacylglycerol kinase zeta; also DAG kinase 6) is a 130 kDa member of the eukaryotic diacylglycerol (DAG) kinase family of proteins. It exists in both cytoplasm and nucleus, and is widely expressed, being found in neurons, skeletal muscle cells and endothelium. DGK- zeta converts DAG into phosphatidic acid (PA). In the nucleus, this activates mTOR and promotes cell proliferation. In the cytoplasm, DGK- zeta-generated PA activates PAK1, leading to increased cell motility. Human DGK- zeta is 1117 amino acids (aa) in length and contains two DAG-type Zn-finger regions (aa 287‑419), a MARCKS homology domain (aa 448‑462) and a catalytic domain (aa 486‑607). In addition to the 130 kDa protein, there are several splice variants that run about 114 kDa in SDS-PAGE.

Long Name

Diacylglycerol Kinase

Alternate Names

DGKZ, DGKzeta

Entrez Gene IDs

8525 (Human); 104418 (Mouse); 81821 (Rat)

Gene Symbol

DGKZ

UniProt

Q13574 (Human)

Additional DGK-zeta Products

Product Documents for Human DGK- zeta Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human DGK- zeta Antibody

For research use only

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