Human DIDO1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6947

R&D Systems, part of Bio-Techne
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MAB6947
MAB6947-SP
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 734823

View all DIDO1 Antibodies »

Product Summary for Human DIDO1 Antibody

Immunogen

E. coli-derived recombinant human DIDO1
Met1-Ser117
Accession # Q9BTC0

Specificity

Detects human DIDO1 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human DIDO1 Antibody

Detection of Human DIDO1 antibody by Western Blot.

Detection of Human DIDO1 by Western Blot.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear (Nuc) extracts. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human DIDO1 Monoclonal Antibody (Catalog # MAB6947) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DIDO1 at approximately 280 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human DIDO1 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Raji human Burkitt's lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DIDO1

DIDO1 (Death-inducer obliterator 1; also DIO-1, C20orf158 and DATF-1) is a 245 kDa (predicted) intracellular protein that belongs the DIO family of proteins.  It is ubiquitous in expression, and apparently serves multiple functions, depending on its splice variant.  The standard form is a nuclear protein that maintains the integrity of mitotic checkpoint protein BubR1.  An absence of DIDO1 results in centrosome amplification and unequal chromatin segregation.  The best known short form (an 83 kDa isoform in mouse), by contrast, induces apoptosis by promoting transcription of caspase 9.  Human standard form DIDO1 is 2240 amino acid (aa) in length.  It contains two NLSs (aa 165-193), one PHD-type Zn finger region (aa 270-320), a TFIIS domain (aa 670-790), an SPOC protein-interaction domain (aa 1057-1163), two Pro-rich segments (aa 1257-1286 and 1725-2034) and a C-terminal Arg-rich region (aa 2108-2214).  There are least 23 utilized phosphoryation sites.  Multiple splice forms exist.  Variants are generated by short aa substitutions for aa 531-2240, 566-2240 and 1182-2240, possibly coupled to a 36 aa insertion after Pro387.  Over aa 1-117, human DIDO1 shares 80% aa identity with mouse DIDO1.

Long Name

Death Inducer Obliterator 1

Alternate Names

BYE1, C20orf158, DATF1, DIO1

Entrez Gene IDs

11083 (Human); 23856 (Mouse); 362286 (Rat)

Gene Symbol

DIDO1

UniProt

Q9BTC0 (Human)

Additional DIDO1 Products

Product Documents for Human DIDO1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human DIDO1 Antibody

For research use only

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