Human Dishevelled-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4216
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 373413
Product Specifications
Immunogen
E. coli-derived recombinant human Dishevelled-2
Leu78-Thr250
Accession # O14641
Leu78-Thr250
Accession # O14641
Specificity
Detects human Dishevelled‑2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) DVL-1 or rhDVL-3 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human Dishevelled-2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human Dishevelled-2
Sample: Recombinant Human Dishevelled-2
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Dishevelled-2
Dishevelled-2 (DVL-2) is a 90‑95 kDa cytoplasmic phosphoprotein that is one of the three Drosophila dishevelled orthologs. Human DVL-2 is expressed in multiple tissues, participates in Wnt signal transduction and may mediate Wnt-dependent motility. DVL-2 is a modular protein containing DIX, PDZ and DEP linker regions that connect Wnt/Frizzled engagement to proteins involved in the inactivation of beta-catenin destruction in the Wnt canonical pathway. Human and mouse DVL-2 share 94% aa identity over the sequence used for immunization.
Alternate Names
Dishevelled2, DVL2
Gene Symbol
DVL2
UniProt
Additional Dishevelled-2 Products
Product Documents for Human Dishevelled-2 Antibody
Product Specific Notices for Human Dishevelled-2 Antibody
For research use only
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