Human DISP2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8434

R&D Systems, part of Bio-Techne
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MAB8434
MAB8434-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 758204

View all DISP2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DISP2
Arg192-Lys477
Accession # A7MBM2

Specificity

Detects human DISP2 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human DISP2 Antibody

Detection of Human DISP2 antibody by Western Blot.

Detection of Human DISP2 by Western Blot.

Western blot shows lysates of NCI-N87 human gastric carcinoma cell line, U-118-MG human glioblastoma/astrocytoma cell line, and COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human DISP2 Monoclonal Antibody (Catalog # MAB8434) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for DISP2 at approximately 152 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human DISP2 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Western blot shows lysates of NCI‑N87 human gastric carcinoma cell line, U‑118‑MG human glioblastoma/astrocytoma cell line, and COLO 205 human colorectal adenocarcinoma cell line.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DISP2

DISP2 (Dispatched Homolog 2), also known as DISPB, belongs to the dispatched family of sterol-sensing proteins.  DISP2 and the closely related DISP1 were initially identified in mice by their high homology with Dispatched, a protein required for long-range Hedgehog (Hh) signaling in Drosophila.  Like DISP1, DISP2 is a multispan (12) transmembrane protein with both cytoplasmic N- and C-terminal tails.  DISP2 contains a sterol-sensing domain (SSD) of 173 amino acids (aa), and may be involved in regulating the release of cholesterol-modified Sonic Hedgehog (Shh).  Human DISP2 is 1401 aa in length, with three predicted N-linked glycosylation sites.  Over aa 192-477, human DISP2 shares 72% and 73% aa identity with mouse and rat DISP2, respectively.

Long Name

Dispatched 2

Alternate Names

DISPB

Entrez Gene IDs

85455 (Human); 214240 (Mouse); 311324 (Rat)

Gene Symbol

DISP2

UniProt

A7MBM2

Additional DISP2 Products

Product Documents for Human DISP2 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human DISP2 Antibody

For research use only

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