Human DMP-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4129

R&D Systems, part of Bio-Techne
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MAB4129
MAB4129-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA Capture (Matched Antibody Pair)

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 560716

View all DMP-1 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human DMP-1 isoform 2
Leu17-Tyr497
Accession # NP_001073380

Specificity

Detects human DMP-1 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant mouse DMP-1, recombinant human (rh) CD44, and rhComplement Factor H is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human DMP-1 Antibody

Human DMP-1 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human DMP-1 Biotinylated Antibody (Catalog # BAM41291)
  • Standard: Recombinant Human DMP-1 Protein, CF (Catalog # 4129-DM)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DMP-1

Dentin matrix protein 1 (DMP-1) is a member of the SIBLING family that also includes bone sialoprotein, dentin sialophosphoprotein, MEPE, and osteopontin. These highly phosphorylated integrin-binding proteins are rich in acidic amino acids and function in the formation of calcified bone and tooth matrix (1, 2). The phosphate content, spacing of acidic residues, and calcium-dependent dimerization of DMP-1 contribute to its ability to sequester calcium phosphate clusters and promote hydroxyapatite (HA) crystal formation (3-5). Rodent DMP-1 is cleaved by BMP-1 family proteases at a single site which is conserved in human, generating a 37 kDa N-terminal and a 57 kDa C-terminal fragment (6). The N-terminal fragment in rat carries chondroitin sulfate (7). The C-terminal fragment alone can nucleate HA crystals, while crystal growth into a needle-like morphology is inhibited by the N-terminal fragment (3, 4). Crystal maturation is dependent on the presence of type I collagen (4). DMP-1 is required for odontoblast differentiation as well as dentin formation (8). Nonphosphorylated DMP-1 is targeted to the nucleus, where it activates the transcription of odontoblast and osteoblast specific genes (9, 10). Early in osteoblast maturation, nuclear DMP-1 is extensively phosphorylated by casein kinase II, triggering its secretion (9). DMP-1 mutations in humans are associated with hypophosphatemia and FGF23 overexpression (11, 12). DMP-1 induces the activation of proMMP-9 and displaces mature MMP-9 from TIMP1 (13). DMP-1 tethering of MMP-9 to the cell surface via CD44 and integrins alphav beta3 and alphav beta5 promotes tumor cell invasiveness in vitro (14). Full length DMP-1 circulates in human serum in a tight complex with complement factor H (13, 14). When first bound to CD44 or integrin alphav beta3, DMP-1 can anchor factor H to the cell surface and protect the cell from complement-mediated lysis (15). Mature human DMP-1 shares 61%-67% amino acid sequence identity with bovine, mouse, and rat DMP-1.

References

  1. Qin, C. et al. (2004) Crit. Rev. Oral Biol. Med. 15:126.
  2. Hirst, K.L. et al. (1997) Genomics 42:38.
  3. He, G. et al. (2003) Nat. Mater. 2:552.
  4. Gajjeraman, S. et al. (2007) J. Biol. Chem. 282:1193.
  5. He, G. et al. (2005) Biochemistry 44:16140.
  6. Steiglitz, B.M. et al. (2004) J. Biol. Chem. 279:980.
  7. Qin, C. et al. (2006 J. Biol. Chem. 281:8034.
  8. Lu, Y. et al. (2007) Dev. Biol. 303:191.
  9. Narayanan, K. et al. (2003) J. Biol. Chem. 278:17500.
  10. Narayanan, K. et al. (2006) J. Biol. Chem. 281:19064.
  11. Lorenz-Depiereux, B. et al. (2006) Nat. Genet. 38:1248.
  12. Feng, J.Q. et al. (2006) Nat. Genet. 38:1310.
  13. Fedarko, N.S. et al. (2004) FASEB J. 18:735.
  14. Karadag, A. et al. (2005) Cancer Res. 65:11545.
  15. Jain, A. et al. (2002) J. Biol. Chem. 277:13700.

Long Name

Dentin Matrix Protein 1

Alternate Names

DMP1

Entrez Gene IDs

1758 (Human); 13406 (Mouse); 25312 (Rat)

Gene Symbol

DMP1

UniProt

NP_001073380

Additional DMP-1 Products

Product Documents for Human DMP-1 Antibody

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Product Specific Notices for Human DMP-1 Antibody

For research use only

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