Human DUX4/DUX4c Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB95351
Recombinant Monoclonal Antibody.
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2143C
Product Specifications
Immunogen
Human DUX4 synthetic peptide containing sequences within amino acids 200-300 of the human DUX4 protein
Accession # Q9UBX2
Accession # Q9UBX2
Specificity
Detects human DUX4/DUX4c in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human DUX4/DUX4c Antibody
Detection of Human DUX4/DUX4c by Western Blot.
Western blot shows lysates of C2C12 mouse myoblast cell line transfected with human DUX4 untreated (-) or treated (+) with 100 ng/mL Doxycycline for overnight. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human DUX4 Monoclonal Antibody (Catalog # MAB95351) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for DUX4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human DUX4/DUX4c Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: C2C12 mouse myoblast cell line transfected with human DUX4 treated with Doxycycline
Sample: C2C12 mouse myoblast cell line transfected with human DUX4 treated with Doxycycline
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DUX4/DUX4c
Human Double homeobox 4 (aka DUX4) is a protein encoded by the DUX4 gene. This gene is located within a D4Z4 repeat array in chromosome 4q35. Each D4Z4 repeat unit has an open reading frame, DUX4, that contains two homeoboxes. Unregulated expression of DUX4 in muscle cells is the cause of facioscapulohumeral muscular dystrophy (FSHD), a common form of muscular dystrophy in adults that is one of the most prevalent genetic diseases of muscle.
Long Name
Double Homeobox 4/Double Homeobox 4c
Alternate Names
DUX10, DUX4, DUX4L
Entrez Gene IDs
22947 (Human)
Gene Symbol
DUX4L1
UniProt
Additional DUX4/DUX4c Products
Product Documents for Human DUX4/DUX4c Antibody
Product Specific Notices for Human DUX4/DUX4c Antibody
For research use only
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