HEK293 human embryonic kidney cell line transfected with (A) EBI2 or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human EBI2 Monoclonal Antibody (Catalog # MAB10272) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based Mouse IgG2A Isotype Control Antibody staining (Catalog # MAB003, data not shown). View our protocol for Staining Membrane-associated Proteins.

Detection of EBI2 in A549 Human Cell Line by Flow Cytometry.

A549 human lung carcinoma cell line was stained with Mouse Anti-Human EBI2 Monoclonal Antibody (Catalog # MAB10272, filled histogram) or Mouse IgG2A Isotype Control Antibody (Catalog # MAB003, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human EBI2 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human EBI2 and eGFP and A549 human lung carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EBI2

Epstein-Barr virus-induced G-protein coupled receptor 2, also known as EBI2 or GPR183 is a G-protein coupled receptor (GPCR) expressed in lymphocytes that acts as a chemotactic receptor for B-cells, T-cells, splenic dendritic cells, and monocytes/macrophages. The GPR183 gene was identified by the up-regulation of its expression upon Epstein-Barr virus infection of the Burkitt's lymphoma cell line BL41. GPR183 collaborates with CXCR5 to mediate B cell homing within a lymph node, probably by forming a heterodimer with CXCR5 that affects its interaction with CXCL13

Long Name

Epstein-Barr Virus Induced Gene 2

Alternate Names

GPR183

Entrez Gene IDs

1880 (Human); 321019 (Mouse); 679975 (Rat)

Gene Symbol

GPR183

UniProt

P32249

Additional EBI2 Products

Product Documents for Human EBI2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human EBI2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human EBI2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10272

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all EBI2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human EBI2 Antibody

Detection of EBI2 in HEK293 Human Cell Line Transfected with Human EBI2 and eGFP by Flow Cytometry.