Human ECE-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB17842
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 303906
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ECE-1
Gln90-Trp770
Accession # P42892
Gln90-Trp770
Accession # P42892
Specificity
Detects human ECE-1 in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Human ECE-1 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney
Sample: Immersion fixed paraffin-embedded sections of human kidney
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ECE-1
Endothelin-converting Enzyme 1 (ECE-1) is a zinc protease of the neprilysin (NEP) family, which also includes ECE-2, PEX, XCE, DINE, Kell and several NEP-like proteins (1). ECE-1 is a type II transmembrane protein with a short cytoplasmic tail and a large ectodomain. Four alternatively spliced isoforms differ in their cytoplasmic tail (2, 3). In addition to big endothelin-1, ECE-1 cleaves a variety of bioactive peptides such as bradykinin, neurotensin, angiotensin I, and substance P (1). Together with ECE-2, it is also involved in degradation of beta-amyloid peptide (4). The ectodomain of human ECE-1, which is common to all isoforms, was expressed with an N-terminal His tag and purified.
References
- Turner, A.J. et al. (2001) BioEssays 23:261.
- Valdennaire, O. et al. (1999) Eur. J. Biochem. 264:341.
- Schweizer, A. et al. (1997) Biochem. J. 328:871.
- Eckman, E.A. et al. (2003) J. Biol. Chem. 278:2081.
Long Name
Endothelin-converting Enzyme-1
Alternate Names
ECE1
Entrez Gene IDs
1889 (Human)
Gene Symbol
ECE1
UniProt
Additional ECE-1 Products
Product Documents for Human ECE-1 Antibody
Product Specific Notices for Human ECE-1 Antibody
For research use only
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