MCF-7 human breast cancer cell line was stained with Rat Anti-Human ECE-1 Monoclonal Antibody (Catalog # MAB17841, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human ECE-1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: MCF-7 human breast cancer cell line fixed with paraformaldehyde and permeabilized with saponin

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ECE-1

Endothelin-converting Enzyme 1 (ECE-1) is a zinc protease of the neprilysin (NEP) family, which also includes ECE-2, PEX, XCE, DINE, Kell and several NEP-like proteins (1). ECE-1 is a type II transmembrane protein with a short cytoplasmic tail and a large ectodomain. Four alternatively spliced isoforms differ in their cytoplasmic tail (2, 3). In addition to big endothelin-1, ECE-1 cleaves a variety of bioactive peptides such as bradykinin, neurotensin, angiotensin I, and substance P (1). Together with ECE-2, it is also involved in degradation of beta-amyloid peptide (4). The ectodomain of human ECE-1, which is common to all isoforms, was expressed with an N-terminal His tag and purified.

References

Turner, A.J. et al. (2001) BioEssays 23:261.
Valdennaire, O. et al. (1999) Eur. J. Biochem. 264:341.
Schweizer, A. et al. (1997) Biochem. J. 328:871.
Eckman, E.A. et al. (2003) J. Biol. Chem. 278:2081.

Long Name

Endothelin-converting Enzyme-1

Alternate Names

ECE1

Entrez Gene IDs

1889 (Human)

Gene Symbol

ECE1

UniProt

P42892

Additional ECE-1 Products

Product Documents for Human ECE-1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human ECE-1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human ECE-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB17841

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all ECE-1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human ECE-1 Antibody

Detection of ECE-1 in MCF-7 Human Cell Line by Flow Cytometry.