Human EDIL3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6046

R&D Systems, part of Bio-Techne
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MAB6046
MAB6046-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 670421

View all EDIL3 Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human EDIL3
Val17-Glu480
Accession # O43854

Specificity

Detects human EDIL3 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human MGF-E8 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human EDIL3 Antibody

Detection of Human EDIL3 antibody by Western Blot.

Detection of Human EDIL3 by Western Blot.

Western blot shows lysates of human lung tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human EDIL3 Monoclonal Antibody (Catalog # MAB6046) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for EDIL3 at approximately 54 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human EDIL3 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Human lung tissue under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EDIL3

EGF-like repeat and discoidin I-like domain-containing protein 3 (EDIL3; also Del-1 and integrin-binding DEL1) is a 52 kDa extracellular matrix protein that is expressed by endothelial tissues during embryonic vascular development (1). Human EDIL3 is synthesized as a precursor with a 16 amino acid (aa) signal sequence and a 464 aa mature chain. The mature chain is composed of three epidermal growth factor (EGF) repeats and two discoidin-I-like domains (1). The second EGF repeat contains an RGD motif (1). Splicing variants produce two isoforms for human EDIL3. Isoform 2 has an A->G substitution at aa 66 and a deletion of aa 67-76 found in isoform 1. Mature human EDIL3 shares 96% aa sequence identity with mature mouse EDIL3. The RGD motif of EDIL3 binds alphav beta5 integrin, which, in turn, leads to increased angiogenic transcription factor HoxD3 expression (2). HoxD3activates alphav beta3 and uPA, resulting in the transformation of resting endothelial cells to an angiogenic state (2-4). EDIL3 becomes quiescent at the time of birth, and is no longer expressed in normal adult tissues. It has been found, however, re-expressed in a number of human tumors as well as in ischemic muscles and ischemic brain tissue, which may play an important role in adult angiogenesis (2, 5-6). EDIL3 promotes adherence and migration of endothelial cells, and acts as an endothelial cell survival agent through upregulation of Bcl-2 expression (7). Exogenous application of EDIL3 has been demonstrated to augment angiogenesis and improve blood flow and tissue function in murine models of hind-limb ischemia (6, 8), cardiac ischemia (9) and cerebral ischemia (2). EDIL3 has also been shown to be an endogenous inhibitor of inflammatory cell recruitment by interfering with the integrin LFA-1-dependent leukocyte-endothelial adhesion (10).

References

  1. Hidai, C. et al. (1998) Genes Dev. 12:21.
  2. Fan, Y. et al. (2008) Brain Res. 1219:1.
  3. Penta, K. et al. (1999) J. Biol. Chem. 274:11101.
  4. Rezaee, M. et al. (2002) Am. J. Physiol. Heart Circ. Physiol. 282:H1924.
  5. Aoka, Y. et al. (2002) Microvasc. Res. 64:148.
  6. Ho, H.K. et al. (2004) Circulation 109:1314.
  7. Pollman, M.J. et al. (1999) J. Cell Physiol. 178:359.
  8. Zhong, J. et al. (2003) J. Clin. Invest. 112:30.
  9. Kown, M.H. et al. (2003) Ann. Thorac. Surg. 76:1246.
  10. Choi, E.Y. et al. (2008) Science 322:1101.

Alternate Names

DEL1

Entrez Gene IDs

10085 (Human); 13612 (Mouse)

Gene Symbol

EDIL3

UniProt

O43854

Additional EDIL3 Products

Product Documents for Human EDIL3 Antibody

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Product Specific Notices for Human EDIL3 Antibody

For research use only

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