Human EDNRA Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6538
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 485711
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human EDRNA
Accession # NP_001948
Accession # NP_001948
Specificity
Detects human EDRNA in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human EDNRA Antibody
EDNRA in Human Kidney.
EDNRA was detected in immersion fixed paraffin-embedded sections of human kidney using Human EDNRA Monoclonal Antibody (Catalog # MAB6538) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human EDNRA Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney
Sample: Immersion fixed paraffin-embedded sections of human kidney
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EDNRA/Endothelin R Type A
EDNRA (endothelin A receptor) is a 427 amino acid (aa) protein of the rhodopsin-like 7-transmembrane receptor family. It can be variably modified by N-linked glycoproteins, phosphorylation and palmitoylation. It binds endothelins ET-1 and ET-2 preferentially, while EDNRB binds all three forms equally. Developmentally, EDNRA is expressed by cranial neural crest cells and induces facial morphogenesis. It is expressed by adult blood vessel smooth muscle cells, and is primarily responsible for the vasoconstrictor effects of ET-1. Human EDNRA shares 86% aa identity with mouse and rat EDNRA within the combined extracellular portions of the molecule.
Long Name
Endothelin Receptor Type A
Alternate Names
EDNRA, ETAR, ETRA
Gene Symbol
EDNRA
UniProt
Additional EDNRA/Endothelin R Type A Products
Product Documents for Human EDNRA Antibody
Product Specific Notices for Human EDNRA Antibody
For research use only
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