Human eIF4B Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3800
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human eIF4B
Met1-Arg200
Accession # P23588
Met1-Arg200
Accession # P23588
Specificity
Detects human and mouse eIF4B in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human eIF4B Antibody
Detection of Human eIF4B by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human eIF4B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3800) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for eIF4B at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human eIF4B Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: A431 human epithelial carcinoma cell line and Neuro-2A mouse neuroblastoma cell line
Sample: A431 human epithelial carcinoma cell line and Neuro-2A mouse neuroblastoma cell line
Reviewed Applications
Read 1 review rated 5 using AF3800 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: eIF4B
eIF4B (eukaryotic translation initiation factor 4B),an RNA binding protein, is essential for the binding of mRNA to the 43S pre-initiation complex, which consists of the 40S ribosomal subunit bound to a ternary complex of eIF2, GTP, and Met-tRNA stabilized by eIF3. An arginine rich motif (ARM) in the carboxy-terminus of eIF4B binds RNA non-specifically, while a canonical RNA motif (RMM) near the amino terminus binds specifically to 18S rRNA. The simultaneous binding of specific and nonspecific RNA may serve to facilitate the binding of the 40S subunit to the mRNA by serving as a bridge between the 18S rRNA and the mRNA. Besides its binding activity, eIF4B in conjunction with eIF4F, a heterotrimeric protein, stimulates the ATPase and RNA helicase activity of eIF4E.
Long Name
Eukaryotic Translation Initiation Factor 4B
Alternate Names
Eif4a2
Gene Symbol
EIF4B
UniProt
Additional eIF4B Products
Product Documents for Human eIF4B Antibody
Product Specific Notices for Human eIF4B Antibody
For research use only
Loading...
Loading...
Loading...
Loading...