Human ELF3 Antibody

ELF3 in Human Prostate.

ELF3 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human ELF3 Monoclonal Antibody (Catalog # MAB57871) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei of glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human ELF3/ESE-1 by Immunohistochemistry

ELF3, EHF, and TGIF1 enhances the malignant phenotypes of LUAD cells.A, B Inhibition of invasive and migrate activity by knockdown master TFs, ELF3, EHF, and TGIF1. PC-9 cells were transfected with indicated siRNA-pool and subjected to invasion and migration assays (see “Materials and methods”). Invaded and migrated cells were stained with crystal violet and counted (see Fig. S3). Histograms represent the number of invasion or migration. Representative photographs were shown. C Colony assay performed on PC-9 cells transfected with either scramble or siRNA-pool targeting ELF3, EHF, and TGIF1. D Silencing of ELF3, EHF, and TGIF1 increased clonal cell growth in a 3D spheroid culture system. PC-9 cells transfected with scramble or siRNA-pool were cultured as spheroid in DMEM medium and the spheroids was observed after 48 h. E Inhibition of cell migration by knockdown master TFs, ELF3, EHF, and TGIF1. PC-9 cells were transfected with indicated siRNA-pool and subjected to scratch assay (see “Materials and methods”). Representative photographs were taken and migration areas were measured. Statistical analysis was performed by using t tests; data are shown as bar graphs of the mean ± SD. of three independent experiments. Scale bars (white) indicate 250 μm. F The effects of pcDNA3.1-ELF3 and/or pcDNA3.1-EHF co-transfected with TGIF1 siRNA on cell migration and invasiveness in LUAD cells were examined by Transwell assays using a Boyden chamber in the presence or absence of Matrigel, respectively. Histograms represent the number of invasion or migration. Data were representative of two to three separate experiments performed in triplicate. *p < 0.05; **p < 0.01; ***p < 0.001. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33070167), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human ELF3/ESE-1 by Immunohistochemistry

ELF3, EHF, and TGIF1 enhances the malignant phenotypes of LUAD cells.A, B Inhibition of invasive and migrate activity by knockdown master TFs, ELF3, EHF, and TGIF1. PC-9 cells were transfected with indicated siRNA-pool and subjected to invasion and migration assays (see “Materials and methods”). Invaded and migrated cells were stained with crystal violet and counted (see Fig. S3). Histograms represent the number of invasion or migration. Representative photographs were shown. C Colony assay performed on PC-9 cells transfected with either scramble or siRNA-pool targeting ELF3, EHF, and TGIF1. D Silencing of ELF3, EHF, and TGIF1 increased clonal cell growth in a 3D spheroid culture system. PC-9 cells transfected with scramble or siRNA-pool were cultured as spheroid in DMEM medium and the spheroids was observed after 48 h. E Inhibition of cell migration by knockdown master TFs, ELF3, EHF, and TGIF1. PC-9 cells were transfected with indicated siRNA-pool and subjected to scratch assay (see “Materials and methods”). Representative photographs were taken and migration areas were measured. Statistical analysis was performed by using t tests; data are shown as bar graphs of the mean ± SD. of three independent experiments. Scale bars (white) indicate 250 μm. F The effects of pcDNA3.1-ELF3 and/or pcDNA3.1-EHF co-transfected with TGIF1 siRNA on cell migration and invasiveness in LUAD cells were examined by Transwell assays using a Boyden chamber in the presence or absence of Matrigel, respectively. Histograms represent the number of invasion or migration. Data were representative of two to three separate experiments performed in triplicate. *p < 0.05; **p < 0.01; ***p < 0.001. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33070167), licensed under a CC-BY license. Not internally tested by R&D Systems.