HEK293 human embryonic kidney cell line transfected with (A) human ELFN1 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human ELFN1 Monoclonal Antibody (Catalog # MAB10644) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB002). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human ELFN1 Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/mL
Sample: HEK293 Human Cell Line Transfected with Human ELFN1 and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ELFN1

ELFN1 (Extracellular leucine-rich repeat and fibronectin type-III domain-containing protein 1), also known as Protein phosphatase 1 regulatory subunit 28, is a member of the extracellular Leucine-Rich Repeat superfamily (1). Expressed mainly in the nervous system, ELFN1 is a transmembrane protein that inhibits the activity of protein phosphatase 1 (PP1) complexes (2). Mature human ELFN1 consists of a 391 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment and a 389 aa cytoplasmic tail. The ECD includes one fibronectin type-III domain, six leucine-rich repeats (LRR) and one LRR C-terminal (LRRCT) domain. Human ELFN1 shares 90% aa sequence identity with mouse and rat ELFN1. The cytoplasmic tail contains many tyrosine but no other detectable motifs. ELFN1 is strongly expressed in globus pallidus and interneurons in cortex and hippocampus in both developing and adult brains (1). It is also expressed in endocrine and reproductive tissues (1). Given the functions and discrete patterns of many known LRR family proteins, it has been proposed that ELFN1 could serve as a neuronal adhesion molecule and play an integral role in synapse formation and differentiation via the coordination of both pre- and postsynaptic machineries, thereby involved in neurite outgrowth, axon guidance, fasciculation, and synapse formation (3). Recent studies showed that ELFN1 physically anchor metabotropic glutamate receptor 6 (mGluR6) and mGluR7 across retinal and hippocampal synapses (3-4), and can be recruited selectively to all group III mGluRs (mGluR4, mGluR6, mGluR7, and mGluR8) to allosterically modulate these receptors (5).

References

Dolan J. et al. (2007) BMC Genomics 8:320.
Hendrickx, A. et al. (2009) Chem. Biol. 16:365.
Williams, M.E. et al. (2010) Neuron 68:9.
De Wit, J. and Ghosh A. (2016) Nat Rev Neurosci 17:22.
Dunn H. A. et al. (2018) PNAS 115:5022.

Long Name

Extracellular Leucine-Rich Repeat And Fibronectin Type III Domain Containing 2

Alternate Names

PPP1R28

Entrez Gene IDs

392617 (Human); 243312 (Mouse); 288512 (Rat)

Gene Symbol

ELFN1

UniProt

P0C7U0

Additional ELFN1 Products

Product Documents for Human ELFN1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human ELFN1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human ELFN1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10644

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all ELFN1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human ELFN1 Antibody

Detection of ELFN1 in HEK293 Human Cell Line Transfected with Human ELFN1 and eGFP by Flow Cytometry